Hengyuan tells you how to reduce the error probability of ELISA kits - Database & Sql Blog Articles

How can the experimental error probability of an ELISA kit be reduced? While careful handling is essential, there are several other key factors that should not be overlooked. Shanghai Hengyuan Bio has summarized nine important aspects to help improve accuracy and reliability during testing: â–³ Aspect 1: The technician should possess strong laboratory skills and hands-on experience. They need to be proficient in operating lab equipment and have the ability to troubleshoot and analyze problems effectively during experiments. â–³ Aspect 2: Use a calibrated micropipette to minimize human errors. Accuracy in pipetting is crucial for quantitative assays, as even small deviations can significantly affect results. â–³ Aspect 3: Follow the manufacturer's instructions carefully and perform all steps according to the manual. Never mix reagents from different batches, as this may lead to inconsistent results. If needed, conduct trials to optimize procedures before full implementation. â–³ Aspect 4: Ensure thorough washing of the plate. Incomplete washing can cause background staining, leading to false positives. Always use fresh washing buffer and avoid reusing old solutions. â–³ Aspect 5: Control reaction time strictly. Too long or too short a reaction time can both impact the binding of the enzyme conjugate with antigens or antibodies, potentially causing false negatives or positives. â–³ Aspect 6: Check the shelf life of the ELISA kit. Using expired reagents can result in unreliable data. Always verify the expiration date before starting the test. â–³ Aspect 7: Evaluate the performance of the reagent. Before using, run control samples and compare results to ensure stability and accuracy. Only use the reagent if it meets quality standards. â–³ Aspect 8: Monitor the color development time closely. Insufficient time may lead to weak signals (false negative), while excessive time can cause overdevelopment (false positive). Be cautious when interpreting results and consider reagent quality as a possible factor. â–³ Aspect 9: Handle the sample accurately and quickly. Inaccurate sample preparation or delays can affect the enzyme activity and ultimately the final reading. Make sure to add the developer and stop solution precisely to avoid errors. If the sample is slow to process, especially in high temperatures, it may degrade, reducing the effectiveness of the reagent.

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